Alternative Splicing of RIOK3 Engages the Noncanonical NFκB Pathway during Rift Valley Fever Virus Infection

Although the noncanonical NFκB pathway was originally identified as a cellular contributing to lymphoid organogenesis, in past 20 years, its involvement innate immunity has become more appreciated. In particular, been found be activated and even exploited by some RNA viruses during infection. Intriguingly, activation of this shown have role disrupting transcription type 1 interferon (IFN), suggesting rationale for why response could co-opted viruses. Rift Valley fever virus (RVFV) is trisegmented ambisense that poses considerable threat domestic livestock human health. Previously, we showed atypical kinase RIOK3 important mounting an IFN RVFV infection epithelial cells, shortly following with (MP12 strain), mRNA alternatively spliced X2 isoform encodes truncated protein. Alternative splicing inhibitory effect on but also stimulates NFκB-mediated inflammatory response. Here, demonstrate alternative associated suggest (MP12) enhance viral success